Cloning and Characterization of the 𝜻-Carotene Desaturase Gene from Chlorella protothecoides CS-41

Table 1

PCR primers and targeted fragments of the zds gene in C. protothecoides CS-41.

Primers	Primer sequence (5′-3′)	Product size (bp)
Core fragment		413
ZDSF	TCTTTGGCTGCTATTTCAACCTG
ZDSR	ATCGTCACAGTTCAGGAAGC
5′-RACE		691
ZRO1	TTCAGGAAGCCCAGAGCGTAG
ZRI1	TTGCCGCCGAGGAAGAAGC
3′-RACE		1390
ZFO1	ACCTGCTGCTCAAGGACCAC
ZFI1	AGACGTGCGTGAGCTGGACTTC
ZFI2	CCGATTGCTGACTACATCACTGCCA
ZDS-ORF		1755 for cDNA; 4587 for DNA
ZDSF1	ATGCAGGCATCAGCGGTGGCC
ZDSR1	TCACGCAGCCACCGCCTGCT
qRT-PCR		171
ZDSRTF	TGGGCACCTCTCCCGTTG
ZDSRTR	AGTTCAGGAAGCCCAGAGCG
Chlorella β-actin		166
c-actinF	TGATGAAGATCCTCACAGAGC
c-actinR	TCTGGCAGTTCAAAGGTCTT