Synergistic Antitumor Effect of Dichloroacetate in Combination with 5-Fluorouracil in Colorectal Cancer
SW620, LoVo, LS174t, HT29 cells, and 293T noncancerous controls were treated with 10 mM DCA and 20 μM 5-FU alone or in combination for 48 hours, and then pulsed with BrdU. Cells were then harvested and stained, and the numbers of BrdU+ cells were calculated as the mean number of the positive cells in eight different fields of view in one image (magnification, 400X). This calculation was repeated three times. *, compared with control. BrdU+ cells in different drug treatment (%, mean ± SD).
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.