Magnetic Resonance Microscopy Contribution to Interpret High-Resolution Magic Angle Spinning Metabolomic Data of Human Tumor Tissue

Figure 2

HRMAS CPMG (top) and MRM spectroscopy PRESS single-voxel spectra from solution (middle) and tissue (bottom) in each of the samples (A–H). MRM single-voxel location in tissue (red box) and solution (yellow box) is illustrated in the MRM images. Samples G and H were the only samples which underwent HRMAS experiments before MRM. Sample F underwent HRMAS study after MRM. The samples A–E underwent parallel study by HRMAS in other piece from the same biopsies. The resonances assigned in the CPMG HRMAS spectrum of sample G are: mannitol (Man), glycine (Gly), choline and phosphocholine (Cho), creatine (Cr), alanine (Ala), lactate (Lac), and lipids (Lip). For each sample, the MRM images shown are those that better illustrate the tissue shape and position inside the rotor. The MRM images shown for samples A and G belong to RARE sequences and the MRM images for samples B–F and H belong to MDEFT images. Moreover, the contrast in these MRM image was balanced to enhance the differences between the tissue and the solution part.