Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don

Table 1

Effect of direct application of colchicine solutions on the seeds of C. roseus and the number of tetraploid plants regenerated (50 seeds for each treatment).

Concentration (%)	Time (hours)
	12	24	36	48
0	0 (50)	0 (50)	0 (50)	0 (50)
0.05	0 (50)	2 (50)	2 (50)	5 (50)
0.1	3 (50)	7 (50)	9 (50)	3 (50)
0.2	8 (50)	15 (50)	12 (50)	5 (50)
0.3	11 (50)	7 (50)	3 (50)	0 (50)
0.4	0 (50)	0 (50)	0 (50)	0 (50)