Research Article
Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don
Table 2
Genes and their primer sequences used for real-time PCR.
| | accession no. (gene) | Primers | Primer sequences (5′–3′) | Amplicon length (bp) | Annealing temperature (°C) |
| | AM236089.1 (prx1) | rtprx1-f | TAGCTCAAACAACTCGGCCACC | 195 | 62 | | rtprx1-r | GCAGCACTGATGAATCGCACC | | AJ250008.1 (asα) | rtasα-f | GGCGGCGAAGCATGGGAACT | 329 | 59 | | rtasα-r | CCTCGGTCCGCTGG GGTTTC | | X69791.1 (cpr) | rtcpr-f | TCGGCCCTGGTACTGGACTAGC | 306 | 65 | | rtcpr-r | TGGCATCACCGCAGACGTAAAC | L10081.1
(sls) | rtsls-f | CTGCCAGCTTTTGCCATATGC | 191 | 60 | | rtsls-r | GAGTCCATGAGTTCTTCAATAG | | M25151.1 (tdc) | rttdc-f | TCGGCATCTCACCTCAAGTTCT | 207 | 60 | | rttdc-r | TCGGGACATATACAGGCGCTT | | U71605.1 (d4h) | rtd4h-f | TTATGATCGAAAAAGTGAATTA | 223 | 58 | | rtd4h-r | TTTCTTGATGATCTGAGTGCGT | | AF053307.1 (dat) | rtdat-f | CTTCTTCTCATCACGTACCAACTC | 172 | 60 | | rtdat-r | ATACCAAACTCAACGGCCTTAG | | X53602.1 (str) | rtstr-f | GCCTTCACCTTCGATTCAACTG | 287 | 62 | | rtstr-r | GTGGCTAGTTGTGTGGCATACC | | AJ251269.1 (g10h) | rtg10h-f | ATAGCCAGAGCGGAGAAGCG | 163 | 55 | | rtg10h-r | TTTCCCCGCCTCAACCATTA | | AJ251249.1 (orca3) | rtocar3-f | CCGGACCCGTTAGAGTAAACC | 212 | 58 | | rtoca3-r | CGTCTCTTCTTCCTTCCTCCAC | | AJ749993.1 (rps9) | rtrsp9-f | GCGTTTGGATGCTGAGTTGAAG | 257 | 62 | | rtrsp9-r | GGCGCTCAAGGAAGTTCTCTAC |
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