Influence of Hsp90 and HDAC Inhibition and Tubulin Acetylation on Perinuclear Protein Aggregation in Human Retinal Pigment Epithelial Cells
Figure 3
(a) Total proteins (20 g) of whole cell extracts were examined by western blot using antibodies against ubiquitin, Hsp90, Hsp70, acetylated tubulin, and Hsc70 of control (C) cells, and cells exposed to 5 M MG-132 (MG) or 0.25 M geldanamycin (GA) or 1 M trichostatin A (TSA) or 1 M taxol (TAX) or 5 M nocodazole (NOC) for 24 hours. In addition, the cells were treated simultaneously with GA or TSA or TAX or NOC and MG-132 for up to 24 hours and then allowed to recover under the indicated conditions for 24 hours. Hsc70 was used to check the equal loading of proteins. (b) Quantifications of western blots.
