3-months-old male Wistar rats. Studied tissue: liver.
Hypothyroidism was induced by i.p. administration of PTU (1 mg/100 g BW) for 4 weeks together with a weekly i.p. injection of IOP (6 mg/100 g BW). T3 was chronically administered by giving seven daily i.p. injections of 15 g T3/100 g BW to hypothyroid rats, while the control euthyroid and hypothyroid rats received saline injections.
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The whole cell protein content of rat liver was analyzed following T3 administration. Identified proteins were involved in substrates and lipid metabolism, energy metabolism, detoxification of cytotoxic products, calcium homeostasis, amino acid catabolism, and the urea cycle.
3-months-old male Wistar rats. Studied tissue: skeletal muscle.
Hypothyroidism and hyperthyroidism were induced as above (Silvestri et al 2006 [55]).
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The whole-cell protein content of gastrocnemius muscles was analyzed. The differentially expressed proteins unambiguously identified were involved in substrates and energy metabolism, stress response, cell structure, and gene expression.