Reverse Genetics Modification of Cytomegalovirus Antigenicity and Immunogenicity by CD8 T-Cell Epitope Deletion and Insertion

Table 1

Recombinant mCMVs with mutated CD8 T cell epitopes.

Virus mCMV-X	Protein	Replacement	Resulting peptide	Ref.
IE1-L176A	IE1	L176A	168YPHFMPTNA176	[8]
IE1-A176L	IE1	A176L[CTA]	168YPHFMPTNL176	[8]
IE1-A176L∗	IE1	A176L[CTT]	168YPHFMPTNL176	[8]
m164-I175A	m164	I175A	167AGPPRYSRA175	[51]
m164-A175I	m164	A175I	167AGPPRYSRI175	[51]
[IE1−L176A+m164−I175A]	IE1+m164	L176A	168YPHFMPTNA176	[51]
		I175A	167AGPPRYSRA175
[IE1−A176L+m164−A175I]	IE1+m164	A176L	168YPHFMPTNL176	[51]
		A175I	167AGPPRYSRI175
SIINFEKL	m164	167AGPPRYSRI175	167SIINFEKL174	[89]
		→167SIINFEKL174
SIINFEKA	m164	167AGPPRYSRI175	167SIINFEKA174	[89]
		→167SIINFEKA174
Δm06m152-SIINFEKL	m164	167AGPPRYSRI175	167SIINFEKL174	[89]
		→167SIINFEKL174
Δm06m152-SIINFEKA	m164	167AGPPRYSRI175	167SIINFEKA174	[89]
		→167SIINFEKA174