In Vitro Culture Conditions for Maintaining a Complex Population of Human Gastrointestinal Tract Microbiota
Table 3
Quantification of total bacteria, Bacteroidetes and Firmicutes in different inoculum amounts of human feces from 3 individuals. All of the copy numbers were obtained from triplicate real-time PCR analyses. The efficiencies were 91.2% for total bacteria, 72.6% for Bacteroidetes, and 75.9% for Firmicutes.
Individual
Inoculum
Total Bacteria
Bacteroidetes
Firmicutes
amount
Log10 copies mL−1a
Log10 copies mL−1a
Log10 copies mL−1a
0 hr
18 hr
0 hr
18 hr
0 hr
18 hr
A
1%
7.67 ± 1.22
8.75 ± 81.16
7.56 ± 0.49
7.89 ± 0.34
7.13 ± 0.35
7.74 ± 0.26
2%
8.21 ± 0.68
8.78 ± 0.74
7.79 ± 0.21
8.09 ± 0.19
7.83 ± 0.19
8.04 ± 0.24
3%
8.30 ± 0.26
8.74 ± 0.52
7.71 ± 0.17
8.16 ± 0.06
7.98 ± 0.32
8.15 ± 0.13
4%
8.72 ± 0.39
9.12 ± 0.21
8.12 ± 0.21
8.59 ± 0.43
8.36 ± 0.21
8.53 ± 0.28
5%
8.86 ± 0.14
9.11 ± 0.32
8.18 ± 0.11
8.48 ± 0.26
8.39 ± 0.18
8.52 ± 0.31
B
1%
8.80 ± 0.14
8.92 ± 0.10
8.16 ± 0.61
8.52 ± 0.92
8.37 ± 0.17
8.51 ± 0.17
2%
9.22 ± 0.18
9.26 ± 0.10
8.38 ± 0.04
8.62 ± 0.46
8.88 ± 0.23
8.94 ± 0.10
3%
9.35 ± 0.15
9.47 ± 0.10
8.59 ± 0.08
8.77 ± 0.12
9.05 ± 0.07
9.12 ± 0.58
4%
9.42 ± 0.27
9.50 ± 0.10
8.74 ± 0.21
8.83 ± 0.59
9.14 ± 0.38
9.24 ± 0.06
5%
9.34 ± 0.10
9.63 ± 0.12
8.36 ± 0.08
8.46 ± 0.21
9.03 ± 0.03
9.40 ± 0.11
C
1%
8.32 ± 0.14
9.28 ± 0.19
8.13 ± 0.30
8.73 ± 0.56
7.77 ± 0.17
8.11 ± 0.24
2%
8.74 ± 0.24
9.37 ± 0.18
8.46 ± 0.05
8.76 ± 0.17
8.37 ± 0.18
8.52 ± 0.13
3%
8.79 ± 0.25
9.36 ± 0.16
8.48 ± 0.20
8.93 ± 0.74
8.36 ± 0.29
8.78 ± 0.15
4%
8.80 ± 0.14
9.42 ± 0.20
8.78 ± 0.12
9.27 ± 0.17
8.29 ± 0.27
8.74 ± 0.10
5%
9.11 ± 0.14
9.50 ± 0.14
8.82 ± 0.01
9.23 ± 0.12
8.76 ± 0.14
9.10 ± 0.13
aData are expressed as the means and standard deviation from samples.
