Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1⁻-hVEGF₁₆₅ by Ultrasound-Targeted Microbubble Destruction

Table 1

Protein expression of VEGF₁₆₅ in mesenchymal stem cells supernatant after transfection (𝑛=25, ng/mL).

Groups	Protein expression of VEGF165
	24 h	48 h	72 h
(1) The blank control group	12.5±1.8	12.1±0.6	11.8±0.1
(2) The control group A	73.1±0.4	74.0±1.2	70.4±1.0
(3) The control group B	67.3±2.1	79.4±0.8	74.1±1.5
(4) The control group C	63.7±2.6	82.1±1.7	76.3±1.3
(5) The UTMD group	218.6±0.9 *	269.2±2.2 *	199.4±2.1 *

* 𝑃<.05, versus each other non-UTMD groups.
(1) The blank control group: MSCs with culture fluid.
(2) The control group A: 4 μg pcDNA3.1⁻-hVEGF₁₆₅ recombinant were transfected into MSCs.
(3) The control group B: 4 μg pcDNA3.1⁻-hVEGF₁₆₅ recombinant mixed with 300 μl SonoVue microbubble were transfected into MSCs.
(4) The control group C: 4 μg pcDNA3.1⁻-hVEGF₁₆₅ recombinant were transfected into MSCs by ultrasonic exposure (illustrated by the example of Mechanic index (MI) 1.0 and exposure time (ET) 60 s).
(5) The UTMD group: 4μg pcDNA3.1⁻-hVEGF₁₆₅ recombinant were transfected into MSCs by UTMD (MI 1.0, ET 60 s).