Functions of MDMX in the Modulation of the p53-Response

Figure 4

Expression of Mdmx-S protein is much lower compared to full-length Mdmx and increased upon MG132 treatment, whereas mRNA levels are even higher in transfection studies. MCF7 cells were transfected with the indicated constructs (6-well plates; 1 μg of each construct/well; all well were also transfected with 250 ng of CMV-eGFP expression vector as transfection control). Twenty four  hrs after transfection, the cells were either mock treated or treated with 20 μM MG132 for 7 hours. Cell lysates were analyzed by Western Blotting with the indicated antibodies (6658 = MDMX-S specific antibody) and extracted RNA was analyzed by quantitative real-time RT-PCR.