Experimental Limitations Using Reprogrammed Cells for Hematopoietic Differentiation

Table 1

Differentiation of MBM- and MEF-derived iPSC lines in comparison to ESC lines. Numbers of cells indicate those developed from 4×10³ undifferentiated cells (day 0). Expression levels represent amounts of mRNA determined by quantitative RT-PCR, normalized to GAPDH expression, and calculated as expression values of the respective genes in undifferentiated Bruce4 ES cells (day 0).

Cell line	Number of Flk1^+ cells on day 5	Number of CD45^+ cells on day 10	Sox-2 expression on day 5 relative to Bruce 4 on day 0	Sox-2 expression on day 10 relative to Bruce 4 on day 0	Oct-4 expression on day 5 relative to Bruce 4 on day 0	Oct-4 expression on day 10 relative to Bruce 4 on day 0	Klf-4 expression on day 5 relative to Bruce 4 on day 0	Klf-4 expression on day 10 relative to Bruce 4 on day 0
	×10^5	×10^5	×10^−2	×10^−2	×10^−2	×10^−2	×10^−2	×10^−2
J1 ES	2.7	5.5	1.3	0.2	31	0.2	480	1.7
Bruce 4 ES	3.2	23	0.5	0.04	41	0.08	32	0.04
MBM-iPS A	3.1	10	1.0	6.3	2600	120	3600	83
MBM-iPS C	2.2	0.1	18	1050	11000	2500	3700	700
MEF-iPS 1	2.6	7.3	16	21	12	56	3.2	5.8
MEF-iPS 5	4.0	18	45	71	0.65	70	22	5.6