Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome: The Differentiation Factors Characterization for Retinal Ganglion Cell line

Table 1

Proteins identified in HNPE conditioned SF-medium by proteomic analyses.

Serial No.	SwissProt No.	Protein name	MW	Score	Subcellular location	Sequence coverage	Molecular function	Biological process
1-1	P02751	Fibronectin precursor	262442	1728	Secreted	25%	Collagen/heparin binding	cell adhesion/migration
1-2	Q08378	Golgin subfamily A member 3	167252	37	Cytoplasm	4%	Transporter activity	Intra-Golgi vesicle-mediated transport
	P02751	Fibronectin precursor	262442	810	Secreted	11%	Collagen/heparin binding	Cell adhesion/migration
	P02452	Collagen α-1(I) chain precursor	138799	163	Secreted	3%	Protein binding	Skeletal/epidermis development
	P11047	Laminin γ-1 chain precursor	177492	74	Secreted	2%	Extracellular matrix structural constituent	Cell adhesion/migration
	P07996	Thrombospondin-1 precursor	129330	50	Secreted	1%	Signal transducer activity	Multicellular organismal development
	O95239	Chromosome-associated kinesin KIF4A	139794	46	Nucleus	2%	Protein binding	Anterograde axon cargo transport
	Q5VTR2	Ubiquitin-protein ligase BRE1A	113592	39	Nucleus	1%	Transcription coactivator activity, ubiquitin-protein ligase activity and binding, zinc ion binding	Regulation of gene-specific transcription, protein polyubiquitination, negative regulation of cell migration
1-3	Q8TF76	Serine/threonine-protein kinase Haspin	88405	36	Nucleus	6%	ATP binding/protein kinase activity	Protein amino acid phosphorylation
	P07996	Thrombospondin-1 precursor	129330	427	Secreted	11%	Signal transducer activity	Multicellular organismal development
	P02452	Collagen α-1(I) chain precursor	138799	231	Secreted	8%	Protein binding	Epidermis/ skeletal development
	P01024	Complement C3 precursor	187046	145	Secreted	2%	Receptor binding	Complement activation
	P02751	Fibronectin precursor	262442	86	Secreted	3%	Collagen/heparin binding	Cell adhesion/migration
	Q14980	Nuclear mitotic apparatus protein 1	238130	50	Nucleus	4%	Protein binding/structural molecule activity	Mitotic anaphase
	P35442	Thrombospondin-2 precursor	129872	48	Secreted	1%	Heparin binding
	P07814	Bifunctional aminoacyl-tRNA synthetase	162923	39	Cytoplasm	1%	Protein binding	Protein complex assembly
	P81274	G-protein signaling modulator 2	75798	56	Cytoplasm	5%	Identical protein binding	G-protein coupled receptor protein signaling pathway
	O14686	Myeloid/lymphoid or mixed-lineage leukemia protein 2	563831	48	Nucleus	1%	Protein/DNA binding	Regulation of transcription, DNA-dependent
	Q9UQ26	Regulating synaptic membrane exocytosis protein 2	160303	43	Cell membrane	2%	Zinc ion binding, Rab GTPase binding	Intracellular protein transport
	Q9UM54	Myosin-6	148620	35	Golgi apparatus	4%	ADP/calmodulin binding	DNA damage response, intracellular protein transport
	O15020	Spectrin β chain, brain 2	271127	38	Cytoplasm, cytoskeleton	2%	Actin binding	Vesicle-mediated transport
1-4	O00339	Matrilin-2 precursor	106768	109	Secreted	3%	Calcium ion binding
1-5	Q08380	Galectin-3-binding protein precursor	65289	404	Secreted	23%	Protein binding/scavenger receptor activity	Cellular defense response/signal transduction
	O94985	Calsyntenin-1 precursor	109724	55	Endoplasmic reticulum membrane, nucleus, Golgi membrane	1%	Calcium ion binding, protein binding	Homophilic cell adhesion
	P13569	Cystic fibrosis transmembrane conductance regulator	168066	37	Membrane	1%	ATP-binding and phosphorylation-dependent chloride channel activity	Respiratory gaseous exchange, transport
	P12814	α-actinin-1	102993	168	Cytoplasm	7%	Integrin binding	Regulation of apoptosis
1-6	O43707	α-actinin-4	104788	544	Nucleus	11%	Integrin binding	Regulation of apoptosis
	P12814	α-actinin-1	102993	521	Cytoplasm	14%	Integrin binding	Regulation of apoptosis
	Q08380	Galectin-3-binding protein precursor	65289	467	Secreted	18%	Protein binding/scavenger receptor activity	Cellular defense response/signal transduction
	P35609	α-actinin-2	103788	165	Cytoplasm	6%	Integrin binding	Regulation of apoptosis
	P34932	Heat shock 70 kDa protein 4	94240	82	Cytoplasm	4%	ATP binding	Response to unfolded protein
	P35711	Transcription factor SOX-5	83973	40	Nucleus	8%	Transcription factor activity	Transcription from RNA polymerase II promoter
1-7	Q08380	Galectin-3-binding protein precursor	65289	277	Secreted	15%	Protein binding/scavenger receptor activity	cellular defense response/signal transduction
	O43707	α-actinin-4	104788	163	Nucleus	10%	Integrin binding	Regulation of apoptosis
	P08238	Heat shock protein HSP 90-β	83081	159	Cytoplasm	5%	Nitric-oxide synthase regulator activity	Response to unfolded protein
	P29400	Collagen α-5(IV) chain precursor	160943	47	Secreted	4%	Binding, extracellular matrix structural constituent
	Q13740	CD166 antigen precursor	65091	40	Membrane	4%	Receptor binding	Cell adhesion/signal transduction
	P07900	Heat shock protein HSP 90- α	84476	225	Cytoplasm	5%	ATP binding/nitric-oxide synthase regulator activity	Response to unfolded protein/signal transduction
	P12814	α-actinin-1	102993	194	Cytoplasm	6%	Integrin binding	Regulation of apoptosis
	P35609	α-actinin-2	103788	74	Cytoplasm	3%	Integrin binding	Regulation of apoptosis
	O00469	Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 precursor	84632	48	Rough endoplasmic reticulum membrane	4%	Protein binding	Protein modification process/response to hypoxia
	P34932	Heat shock 70 kDa protein 4	94240	44	Cytoplasm	3%	ATP binding	Response to unfolded protein
	Q13045	Protein flightless-1 homolog	144659	43	Nucleus	3%	Actin binding	Muscle contraction
1-8	P02768	Serum albumin precursor	69321	87	Secreted	3%	Protein binding/pyridoxal phosphate binding	Negative regulation of apoptosis /transport
	Q8TEU7	Rap guanine nucleotide exchange factor 6	179294	44	Cytoplasm	3%	GTP-dependent protein binding	Ras protein signal transduction
1-9	O95248	SET-binding factor 1	208125	41	Nucleus	1%	Protein tyrosine/serine/threonine phosphatase activity	Protein amino acid dephosphorylation
	Q9UPQ9	Trinucleotide repeat-containing 6B protein	182703	36	Cytoplasmic mRNA processing body	2%	RNA binding, nucleotide binding	Gene silencing by RNA, regulation of translation
	P29401	Transketolase	67835	134	Cytosol	7%	Protein binding	Transketolase activity
	P02768	Serum albumin precursor	69321	82	Secreted	5%	Protein binding/pyridoxal phosphate binding	Negative regulation of apoptosis/transport
	O60333	Kinesin-like protein KIF1B	204305	58	Cytoplasmic vesicle	1%	ATPase activity/microtubule motor activity	Neuromuscular synaptic transmission/nerve-nerve synaptic transmission
	O75095	Multiple epidermal growth factor-like domains 6 precursor	128524	46		6%	Calcium ion binding
1-10	P06744	Glucose-6-phosphate isomerase	62976	334	Cytoplasm	8%		Humoral immune response/carbohydrate metabolic process
	P27797	Calreticulin precursor	48112	67	Endoplasmic reticulum lumen, Cytoplasm, Secreted	5%	DNA/protein binding	Regulation of apoptosis/transcription, DNA-dependent, protein export from nucleus
	P09493	Tropomyosin 1 α chain	32689	44	Cytoplasm	8%	Structural constituent of muscle	Cell motility, regulation of heart/muscle contraction
1-11	Q06495	Sodium-dependent phosphate transport protein 2A	68893	49	Membrane	1%	Sodium-dependent phosphate transmembrane transporter activity	Body fluid secretion, phosphate transport
	P37268	Squalene synthetase	48084	40	Endoplasmic reticulum membrane	5%	Farnesyl-diphosphate farnesyltransferase activity	Steroid biosynthetic process
	Q12799	T-complex protein 10A homolog	45440	39	Cytosol	7%
	O75095	Multiple epidermal growth factor-like domains 6 precursor	128524	46		1%	Calcium ion binding
	Q9UM47	Neurogenic locus notch homolog protein 3 precursor	243496	37	Cell membrane	5%	Protein binding
	P39191	Alu subfamily SB2 sequence contamination warning entry	65263	59		5%
	Q12841	Follistatin-related protein 1 precursor	34963	52	Secreted	2%	Heparin binding	BMP signaling pathway
	Q14980	Nuclear mitotic apparatus protein 1	238130	41	Nucleus	2%	Protein binding/structural molecule activity	Mitotic anaphase/nuclear organization and biogenesis
1-12	P14136	Glial fibrillary acidic protein, astrocyte	49850	80	Cytoplasm	7%	Structural constituent of cytoskeleton
	P78527	DNA-dependent protein kinase catalytic subunit	468788	39	Nucleus	1%	DNA-dependent protein kinase activity	Peptidyl-serine phosphorylation
	Q9UPQ9	Trinucleotide repeat-containing 6B protein	182703	49		4%
	P06744	Glucose-6-phosphate isomerase	62976	401	Cytoplasm	16%		Humoral immune response/carbohydrate metabolic process
	P14618	Pyruvate kinase isozymes M1/M2	57769	145	Cytosol	7%	Pyruvate kinase activity	Glycolysis
	P00390	Glutathione reductase, mitochondrial precursor	56221	69	Mitochondrion	2%	Glutathione-disulfide reductase activity/electron carrier activity
	P09622	Dihydrolipoyl dehydrogenase, mitochondrial precursor	54116	47	Mitochondrion matrix	4%	Dihydrolipoyl dehydrogenase activity
	P30154	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A β isoform	66159	42		3%	Protein heterodimerization activity
	O95271	Tankyrase-1	141922	37	Cytoplasm	2%	NAD+ ADP-ribosyltransferase activity	Peptidyl-serine/threonine phosphorylation
1-13	P06733	α-enolase	47008	697	Cytoplasm	27%	Transcription factor activity, phosphopyruvate hydratase activity	Negative regulation of cell growth
	Q15113	Procollagen C-endopeptidase enhancer 1 precursor	47942	114	Secreted	10%		Multicellular organismal development
	P13929	β-enolase	46826	242	Cytoplasm	10%	Phosphopyruvate hydratase activity
	P09104	γ-enolase	47108	135	Cytoplasm	4%	Phosphopyruvate hydratase activity
	Q7Z3E2	Protein C10orf118	103623	42		2%
	P11117	Lysosomal acid phosphatase precursor	48285	42	Lysosome	2%	Acid phosphatase activity
	P02686	Myelin basic protein	33097	40	Myelin membrane	7%		Axon ensheathment, immune response, central nervous system development
	P50395	Rab GDP dissociation inhibitor β	50631	40	Cytoplasm	2%	Rab GDP-dissociation inhibitor activity	Signal transduction
	Q13371	Phosducin-like protein	34260	40	Cytoplasm	8%	Regulator of G-protein signaling activity	Signal transduction
	P35573	Glycogen debranching enzyme	174523	39	Cytosol	1%	Amylo-α-1,6-glucosidase activity
	Q6ZU80	Protein C14orf145	73526	38		4%
	P49746	Thrombospondin-3 precursor	104135	36	Secreted	1%	Calcium ion binding	Cell-matrix adhesion
	Q7L1I2	Synaptic vesicle glycoprotein 2B	77393	35	Cytoplasmic vesicle	3%
1-14	P09486	SPARC precursor	34610	36	Secreted	14%	Calcium/calcium ion binding	Ossification, transmembrane receptor protein tyrosine kinase signaling pathway
1-15	P04075	Fructose-bisphosphate aldolase A	39264	341	Cytoskeleton	37%	Fructose-bisphosphate aldolase activity	Fructose metabolic process, glycolysis
	P09972	Fructose-bisphosphate aldolase C	39300	134	Cytoskeleton	13%	Fructose-bisphosphate aldolase activity	Fructose metabolic process
	P00505	Aspartate aminotransferase, mitochondrial precursor	47445	45	Mitochondrion matrix	3%	Aspartate transaminase activity	Fatty acid transport, response to ethanol
1-16	P40925	Malate dehydrogenase, cytoplasmic	36272	40	Cytoplasm	11%	L-malate dehydrogenase activity, malic enzyme activity
	O00623	Peroxisome assembly protein 12	40771	37	Peroxisome membrane	5%	Zinc ion/protein C-terminus binding	Protein import into peroxisome matrix
	Q9NVP4	Protein C20orf12	62868	33	Intracellular	2%	zinc ion binding
	P07195	L-lactate dehydrogenase B chain	36484	52	Cytoplasm	5%	L-lactate dehydrogenase activity
	P10909	Clusterin precursor	52461	42	Secreted	5%	Protein binding	Complement activation, lipid metabolic process
1-17	P11117	Lysosomal acid phosphatase precursor	48285	44	Lysosome	2%	Acid phosphatase activity
	P09493	Tropomyosin 1 α chain	32689	59	Cytoplasm	3%	Structural constituent of muscle	Cell motility, regulation of heart/muscle contraction
1-18	P18669	Phosphoglycerate mutase 1	28655	151	Cytosol	16%	Bisphosphoglycerate 2-phosphatase activity	Glycolysis
	P63104	14-3-3 protein zeta/delta	27728	110	Cytoplasm	12%	Transcription factor binding	Antiapoptosis, signal transduction
	O60242	Brain-specific angiogenesis inhibitor 3 precursor	171379	38	Cell membrane	1%
	O00459	Phosphatidylinositol 3-kinase regulatory subunit β	81574	34	Cytosol	2%	Protein binding	Negative regulation of antiapoptosis
	P46940	Ras GTPase-activating-like protein IQGAP1	189134	34	Cell membrane	2%	GTPase activator/inhibitor activity, calmodulin binding	Signal transduction
1-19	P60174	Triosephosphate isomerase	26522	156	Cytosol	18%	Triose-phosphate isomerase activity
	P26232	α-2 catenin	105085	34	Cytoplasm	1%	Structural constituent of cytoskeleton	Cell adhesion
1-20	P60174	Triosephosphate isomerase	26522	373	Cytosol	37%	Triose-phosphate isomerase activity
	Q06495	Sodium-dependent phosphate transport protein 2A	68893	49	Membrane	1%	Sodium-dependent phosphate transmembrane transporter activity, protein binding	Body fluid secretion, phosphate metabolic process and transport
	Q14980	Nuclear mitotic apparatus protein 1	238115	44	Nucleus	4%	Protein binding, structural molecule activity	Mitotic anaphase, nuclear organization and biogenesis
	P78527	DNA-dependent protein kinase catalytic subunit	468788	36	Nucleus	1%	DNA-dependent protein kinase activity/protein binding	Peptidyl-serine phosphorylation
1-21	Q06830	Peroxiredoxin-1	22096	231	Cytoplasm, melanosome	19%	Peroxidase activity/protein binding	Cell proliferation, hydrogen peroxide catabolic process, skeletal development
	P30086	Phosphatidylethanolamine-binding protein 1	20913	122	Cytoplasm	10%	Phosphatidylethanolamine binding/protein binding
	P16035	Metalloproteinase inhibitor 2 precursor	24383	104	Secreted	13%	Metalloendopeptidase inhibitor activity/protein binding
	P04179	Superoxide dismutase	24707	66	Mitochondrion matrix	6%	Superoxide dismutase activity	Age-dependent response to reactive oxygen species, regulation of transcription from RNA polymerase II promoter, response to superoxide, superoxide metabolic process
	P53618	Coatomer subunit β	107071	51	Cytoplasm	2%	Protein binding	COPI coating of Golgi vesicle, intra-Golgi vesicle-mediated transport, retrograde vesicle-mediated transport, Golgi to ER
	O43395	U4/U6 small nuclear ribonucleoprotein Prp3	77481	45	Nucleus speckle	3%	Protein binding/RNA splicing factor activity, transesterification mechanism	Nuclear mRNA splicing, via spliceosome
	O15240	Neurosecretory protein VGF precursor	67247	44	Secreted	7%		Response to cAMP
	Q06495	Sodium-dependent phosphate transport protein 2A	68893	42	Membrane	1%	Protein binding/sodium-dependent phosphate transmembrane transporter activity	Body fluid secretion, phosphate metabolic process, phosphate transport
	Q9Y587	AP-4 complex subunit sigma-1	16994	41	Golgi apparatus	9%	Transporter activity
	Q04760	Lactoylglutathione lyase	20575	40	Cytoplasm	4%	Lactoylglutathione lyase activity	Antiapoptosis, carbohydrate metabolic process
	P11117	Lysosomal acid phosphatase precursor	48285	40	Lysosome	2%	Acid phosphatase activity
	Q9P0K1	ADAM 22 precursor	100368	39	Membrane	5%	Integrin binding	Central nervous system development, negative regulation of cell adhesion
	Q96Q42	Alsin	183550	38	Cytosol	1%	Protein homodimerization activity/protein serine/threonine kinase activator activity/Rab GTPase binding/Rac guanyl-nucleotide exchange factor activity	Cell death, endosome organization, neuron projection morphogenesis, positive regulation of Rac GTPase activity, positive regulation of Rac protein signal transduction, positive regulation of protein kinase activity, regulation of endosome size
1-22	Q06830	Peroxiredoxin-1	22096	110	Cytoplasm, Melanosome	21%	Peroxidase activity/protein binding	Cell proliferation, hydrogen peroxide catabolic process, skeletal development
	Q8N1I0	Dedicator of cytokinesis protein 4	225005	41	Intracytoplasmic membrane	1%	PDZ domain binding/Rac GTPase activator activity/Rac GTPase binding
	P11117	Lysosomal acid phosphatase precursor	48285	39	Lysosome	2%	Acid phosphatase activity
1-23	O95274	Ly6/PLAUR domain-containing protein 3 precursor	35948	38	Cell membrane	8%
	P00441	Superoxide dismutase [Cu-Zn]	15795	73	Cytoplasm, cytosol, nucleus	4%	Chaperone/phosphatase 2B/copper ion/zinc ionbinding, protein homodimerization activity, superoxide dismutase activity/antioxidant activity	Cell aging, oxidation reduction, regulation of organ growth, positive regulation of apoptosis
	O15516	Circadian locomoter output cycles protein kaput	95244	55	Cytoplasm, nucleus	1%	Transcription factor activity	Circadian rhythm, photoperiodism, positive regulation of transcription from RNA polymerase II promoter, signal transduction
	O00160	Myosin If	124725	39		2%	Actin binding/ATP binding/calmodulin binding
	Q13075	Baculoviral IAP repeat-containing protein 1	159479	39	Intracellular	1%	Nucleoside-triphosphatase activity, nucleotide binding, zinc ion binding	Antiapoptosis, nervous system development
1-24	Q6ZUB1	Protein C9orf79	157037	35	Membrane	1%
	Q9BXM0	Periaxin	154906	35	cytoplasm, nucleus	1%	Protein binding	Axon ensheathment
	Q15084	Protein disulfide-isomerase A6 precursor	48091	38	Endoplasmic reticulum lumen, melanosome	2%	Protein binding/protein disulfide isomerase activity	Protein folding
	Q13136	Liprin-α-1	135695	42	Cytoplasm	2%	Protein binding/signal transducer activity	Cell-matrix adhesion, signal transduction
	Q32MQ0	Protein ZNF750	77312	44	Intracellular	5%	Zinc ion binding
	Q15113	Procollagen C-endopeptidase enhancer 1 precursor	47942	48	Secreted	3%		Multicellular organismal development
	P15531	Nucleoside diphosphate kinase A	17138	67	Cytoplasm, nucleus	17%	Deoxyribonuclease activity, DNA/GTP/magnesium ion binding, nucleoside diphosphate kinase activity	Negative regulation of cell proliferation, positive regulation of DNA binding, positive regulation of epithelial cell proliferation, regulation of apoptosis
	P00441	Superoxide dismutase [Cu-Zn]	15795	70	Cytoplasm, cytosol, nucleus	19%	Chaperone/phosphatase 2B/copper ion/zinc ionbinding, protein homodimerization activity, superoxide dismutase activity/antioxidant activity	Cell aging, oxidation reduction, regulation of organ growth, positive regulation of apoptosis
	P23528	Cofilin-1	18360	139	Nucleus matrix, cytoplasm	17%	Protein binding	Actin cytoskeleton organization and biogenesis, antiapoptosis, Rho protein signal transduction
1-25	Q96EZ8	Microspherule protein 1	51771	35	Nucleus	2%	Protein binding	Protein modification process
	O95271	Tankyrase 1	141922	35	Cytoplasm, Golgi apparatus membrane, nucleus	1%	NAD+ ADP-ribosyltransferase activity	Peptidyl-serine phosphorylation, peptidyl-threonine phosphorylation, telomere maintenance via telomerase
	Q14980	Nuclear mitotic apparatus protein 1	238115	35	Nucleus	1%	Protein binding, structural molecule activity	Mitotic anaphase, nuclear organization and biogenesis
	P17612	cAMP-dependent protein kinase, α-catalytic subunit	40433	37	Cytoplasm, nucleus	1%	ATP binding, cAMP-dependent protein kinase activity	Hormone-mediated signaling, protein amino acid phosphorylation, protein kinase cascade
	P11117	Lysosomal acid phosphatase precursor	48285	39	Lysosome	2%	acid phosphatase activity
	P10071	Zinc finger protein GLI3	169743	39	Nucleus	1%	Protein binding, transcription factor activity	Anatomical structure morphogenesis, multicellular organismal development, protein import into nucleus, translocation, signal transduction
	P78527	DNA-dependent protein kinase catalytic subunit	468788	40	Nucleus	1%	DNA-dependent protein kinase activity	Peptidyl-serine phosphorylation
	Q06495	Sodium-dependent phosphate transport protein 2A	68893	40	Membrane	1%	Protein binding, sodium-dependent phosphate transmembrane transporter activity	Body fluid secretion, phosphate metabolic process, phosphate transport
	P15531	Nucleoside diphosphate kinase A	17138	86	Cytoplasm, nucleus	17%	Deoxyribonuclease activity, DNA/GTP/magnesium ion binding, nucleoside diphosphate kinase activity	Negative regulation of cell proliferation, positive regulation of DNA binding, positive regulation of epithelial cell proliferation, regulation of apoptosis
	P62937	Peptidyl-prolyl cis-trans isomerase A	17870	422	Cytoplasm	37%	Unfolded protein binding, virion binding	Initiation of viral infection, protein folding, provirus integration, regulation of viral genome replication
1-26	P62937	Peptidyl-prolyl cis-trans isomerase A	17870	171	Cytoplasm	32%	Unfolded protein binding, virion binding	Initiation of viral infection, protein folding, provirus integration, regulation of viral genome replication
	O95352	Autophagy-related protein 7	77909	34	Cytoplasm	2%	Protein homodimerization activity, ubiquitin activating enzyme activity	Membrane fusion, positive regulation of protein modification process, protein amino acid lipidation
1-27	Q9UPT6	C-jun-amino-terminal kinase-interacting protein 3	146962	54	Cytoplasm	2%	kinesin/protein kinase binding, MAP-kinase scaffold activity	Regulation of JNK cascade, vesicle-mediated transport
	Q8IWJ2	GRIP and coiled-coil domain-containing protein 2	184545	38	Cytoplasm, Golgi apparatus membrane	1%	Identical protein binding
	Q9NX63	Coiled-coil-helix-coiled-coil-helix domain-containing protein 3	26136	39	Mitochondrion	13%	Protein binding
	P02452	Collagen α-1(I) chain precursor	138799	44	Secreted	3%	Protein binding	Epidermis development, skeletal development
	P59998	Actin-related protein 2/3 complex subunit 4	19523	45	Cytoplasm	2%	Protein binding, structural constituent of cytoskeleton	Actin nucleation
	P01034	Cystatin C precursor	15789	56	Secreted	7%	Cysteine protease inhibitor activity, protein homodimerization activity
	P23528	Cofilin-1	18360	178	Nucleus matrix, cytoplasm	19%	Protein binding	Actin cytoskeleton organization and biogenesis, antiapoptosis, Rho protein signal transduction
	P07737	Profilin-1	14914	478	Cytoplasm	45%	Actin binding, proline-rich region binding	Actin cytoskeleton organization
1-28	P09382	Galectin-1	14575	41	Cytoplasm	7%	Protein binding, signal transducer activity	Positive regulation of I-κB kinase, NF-κB cascade, regulation of apoptosis
	P10599	Thioredoxin	11599	59	Cytoplasm	13%	Protein binding	Cell motility, cell proliferation, cell-cell signaling, signal transduction
	P04080	Cystatin B	11133	61	Cytoplasm, nucleus	12%	Endopeptidase inhibitor activity, protein binding
	P07737	Profilin-1	14914	320	Cytoplasm	42%	Actin binding, proline-rich region binding	Actin cytoskeleton organization
1-29	Q9UPQ9	Trinucleotide repeat-containing 6B protein	182703	34	Cytoplasmic mRNA processing body	4%	RNA binding, nucleotide binding	Gene silencing by RNA, regulation of translation
	P78527	DNA-dependent protein kinase catalytic subunit	468788	35	Nucleus	1%	DNA-dependent protein kinase activity, protein binding	Peptidyl-serine phosphorylation
	P81605	Dermcidin precursor	11277	45	Secreted	12%	Protein binding
	P10599	Thioredoxin	11599	76	Cytoplasm	19%	Protein binding	Cell motility, cell proliferation, cell-cell signaling, signal transduction
1-30	Q6NUM9	All-trans-retinol 13,14-reductase precursor	66777	39	Endoplasmic reticulum membrane	3%	All-trans-retinol 13,14-reductase activity	Oxidation reduction, retinol metabolic process
	P14174	Macrophage migration inhibitory factor	12337	39	Cytoplasm, secreted	11%	Cytokine activity	Cell proliferation, cell surface receptor linked signal transduction, negative regulation of apoptosis, prostaglandin biosynthetic process, regulation of macrophage activation
	Q6KC79	Nipped-B-like protein	315854	36	Nucleus	1%	Protein C-terminus binding	Maintenance of mitotic sister chromatid cohesion
	P82279	Crumbs homolog 1 precursor	154081	36	Secreted	1%	Calcium ion binding	Cell-cell signaling, establishment and/or maintenance of cell polarity, response to stimulus
	Q9NX63	Coiled-coil-helix-coiled-coil-helix domain-containing protein 3	26136	39	Mitochondrion	13%	Protein binding
	Q8N0X7	Spartin	72788	41		2%		Cell death
	P14174	Macrophage migration inhibitory factor	12337	60	Cytoplasm, secreted	5%	Cytokine activity	Cell proliferation, cell surface receptor linked signal transduction, negative regulation of apoptosis, prostaglandin biosynthetic process, regulation of macrophage activation
	P58546	Myotrophin	12756	71	Cytoplasm	7%	Protein binding	Cell growth, neuron differentiation
1-31	Q8WUT4	Uncharacterized protein C20orf75 precursor	78794	37	Membrane	5%	Protein binding
	O94851	Protein MICAL-2	126609	40	Cytoplasm	1%	Monooxygenase activity, zinc ion binding	Metabolic process
	Q9Y333	U6 snRNA-associated Sm-like protein LSm2	10828	69	Nucleus	20%	Protein/U6 snRNA binding	Nuclear mRNA splicing

These serial numbers are designated as in Figure 3.
Swiss-Prot/TrEMBL accession number was given from http://us.expasy.org/.