Methotrexate Toxicity in Growing Long Bones of Young Rats: A Model for Studying Cancer Chemotherapy-Induced Bone Growth Defects in Children
Figure 1
Effect of acute high-dose MTX chemotherapy on growth plate structure and cellular changes in young rats. H & E stained section of a normal rat tibial growth plate (a) and an MTX-treated rat growth plate (b). Dashed line represents total heights of growth plates. BrdU labeling showing proliferative chondrocytes in a normal rat (c) and an MTX-treated rat (d), with arrows pointing to proliferating chondrocytes. Normal proliferative/hypertrophic chondrocytes of a normal rat (e) showing no apoptosis; MTX-treated rats with apoptotic chondrocytes in lower proliferative/upper hypertrophic zone (f), and a magnified view of apoptotic chondrocyte (g). (Images are from the authors’ own lab and have not been published previously.)
