Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform
Figure 5
Binding assay by cosedimentation of F-actin with UT12 and BSA for control. 10% SDS-PAGE with protein staining by Coomassie brilliant blue R250. Pellet (P) and supernatant (S) fractions with different molar ratios of UT12 (a) or BSA (b) to actin in the presence of 2 mM MgCl2 and 0.5 mM CaCl2. UT12 and BSA varied from 0.6 up to 19 μM. Actin was held constant at 6 μM. The positions of UT12, BSA, and actin are indicated at the left. Arrows point to unbound UT12 in the supernatant. At all concentrations, no BSA was bound to actin.
