Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform

Table 2

Content of full-length utrophin in tissues from young (2–8 days of age) and adult (6–8 weeks old) rats. Averages of densitometric evaluation of immunoblots (SEM is given for averages from 5 experiments or more; number 𝑛 of experiments in brackets). Values of adult liver are set at 100. For comparison, averages of 3 experiments are given for aorta and uterus. Relative band intensities are always determined in runs together with adult liver and/or heart for calibration. For experimental details, see the Methods section.

Tissue	Young		Adult
Liver	(5)	117 ± 24*	(17)	100
Brain	(5)	139 ± 22*	(5)	105 ± 13*
Kidney	(5)	229 ± 29	(6)	278 ± 27
Heart	(8)	362 ± 38	(11)	299 ± 14
Skeletal muscle	(12)	211 ± 18	(20)	21.0 ± 1.5**
Aorta		—	(3)	569
Uterus		—	(3)	539

(*) Significantly lower (𝑃<0.002) than young and adult heart and adult kidney.  (**)Significantly lower (𝑃<0.0001) than any other tissue with 𝑛 of 5 or higher.