Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome
Figure 6
ORF11 is not required for viral infection in Balb/c mice. (a) The acute lung viral titers. Balb/c mice were intranasally inoculated with 20 PFU of each virus. The lungs were harvested at day 9 after infection and the titers were determined by plaque assays. Shown are the means of viral titers from 3 mice per virus infection and the standard errors of the means. (b) The viral latency in spleens. 20 PFU of each virus was inoculated into Balb/c mice. The spleens were harvested at day 15 after infection and the amounts of latent virus were determined by infective center assays. (c) The copy numbers of viral genomes in the spleens harvested at day 15 after infection. 50 ng of splenic DNA was used for real-time PCR to determine the viral DNA copy numbers. Shown are the means from 3 mice per virus infection and the standard errors of the means.
