Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome

Table 1

Sequences of the primers.

Primer name	Sequence	Position (MHV-68 sequence)
loxP4	GATCCATAACTTCGTATAATGTATGCTATA
	CGAAGTTAT TTAAATATCGATGCTAGCG
loxP5	CCGCTAGCATCGATTTAAAT AACTTCGTAT
	AGCATACATTATACGAAGTTATG
AF	TCTCCTCGAGggttgaatgcatcttgtagg	59095–59114
AR	GGATCCTTAATTAATTGACCGGgcctcaccgctcatctgaaa	59479–59498
BF	CCGGTCAATTAATTAAGGATCCtgggtagatccaggagagat	59499–59518
BR	CTATGCTAGCgatctagcaaacctctggga	59948–59929

The non-MHV-68 sequences of primers are in caps. The italic indicates the engineered restriction enzymes. For loxP4 and loxP5, NheI, ClaI, and SwaI were added next to the loxP sequence (underlined) for cloning purposes. For AF and BR, XhoI and NheI sites were introduced into the primers, respectively, for cloning into pGS284. For AR and BF, BamHI and PacI were included in the primers for screening purposes.