Figure 6: DMSO induces effector T cell apoptotic death in vitro. OT-I splenocytes were prepared and incubated with indicated concentrations of DMSO (ranging 1.563%–25%) for 2 hours. Cells were then double stained with FITC-annexin V and propidium iodide (PI) and analyzed by flow cytometry. Gate was set on lymphocytes according to scatter criteria. The percent of single- and double-stained cells is indicated. The results are representative of two separate experiments.