Cervicovaginal Safety of the Formulated, Biguanide-Based Human Immunodeficiency Virus Type 1 (HIV-1) Inhibitor NB325 in a Murine Model

Figure 2

Exposure to N-9 results in severe damage to cervical epithelial tissues. Mice were exposed to N-9 and sacrificed at 10 min, 2 h, 4 h, 8 h, or 24 h after application. H&E-stained vaginal ((a), (c), (e)) and cervical ((b), (d), (f)) epithelial tissues were analyzed for damage. Representative micrograph fields from the 2 h, 4 h, and 8 h exposures are shown. Images are representative of three mice per exposure duration. Tissues exposed to N-9 for 10 min and 24 h were similar to saline-exposed tissues (data not shown).