TY - JOUR A2 - Chen, Su S. AU - Zeng, Yili AU - Huang, Qinmiao AU - Zheng, Meizhu AU - Guo, Jianxin AU - Pan, Jingxin PY - 2012 DA - 2012/03/12 TI - Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells SP - 101737 VL - 2012 AB - This study explored the effects of reactive nitrogen metabolites (RNMS) on natural-killer- (NK-) cell-mediated killing of K562 cells and the influence of RNM scavengers, such as tiopronin (TIP), glutamylcysteinylglycine (GSH), and histamine dihydrochloride (DHT), on reversing the suppressing effect of RNM. We administered exogenous and endogenous RNM in the NK + K562 culture system and then added RNM scavengers. The concentrations of RNM, TNF-β and IFN-γ, and NK-cell cytotoxicity (NCC) and the percentage of living NK cells were then examined. We found that both exogenous and endogenous RNM caused the KIR to decrease (P<0.01); however, RNM scavengers such as TIP and GSH rescued this phenomenon dose dependently. In conclusion, our data suggests that RNM scavengers such as TIP and GSH enhance the antineoplasmic activity of NK cells. SN - 2314-6133 UR - https://doi.org/10.1155/2012/101737 DO - 10.1155/2012/101737 JF - Journal of Biomedicine and Biotechnology PB - Hindawi Publishing Corporation KW - ER -