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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 101737, 7 pages
Research Article

Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells

1Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Fujian, Quanzhou 362000, China
2Department of Medicine, Shishi hospital, Fujian, Quanzhou 362000, China

Received 28 October 2011; Accepted 27 December 2011

Academic Editor: Su S. Chen

Copyright © 2012 Yili Zeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study explored the effects of reactive nitrogen metabolites (RNMS) on natural-killer- (NK-) cell-mediated killing of K562 cells and the influence of RNM scavengers, such as tiopronin (TIP), glutamylcysteinylglycine (GSH), and histamine dihydrochloride (DHT), on reversing the suppressing effect of RNM. We administered exogenous and endogenous RNM in the NK + K562 culture system and then added RNM scavengers. The concentrations of RNM, TNF-β and IFN-γ, and NK-cell cytotoxicity (NCC) and the percentage of living NK cells were then examined. We found that both exogenous and endogenous RNM caused the KIR to decrease ( 𝑃 < 0 . 0 1 ); however, RNM scavengers such as TIP and GSH rescued this phenomenon dose dependently. In conclusion, our data suggests that RNM scavengers such as TIP and GSH enhance the antineoplasmic activity of NK cells.