Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants
Figure 2
Results of super pool (SP) screening for the barley BAC library HVVMRXALLeA. (a) Schematic representation of 55 barley super pools originated from the BAC library HVVMRXALLeA [24]. SP1 to SP55 represent the super pools 1 to 55. Each super pool is composed of microtiter plates, that is, 2688 BACs per super pool. NEG: negative control. POS: positive control, in this case genomic DNA of the barley cultivar Morex where the BAC library was derived. (b) Ethidium-bromide-stained agarose gel (1.5%) image of the super pool screening. Only the positive super pools (highlighted with a green border in (a)) amplified a single fragment that is of identical size to the product in Morex. Arrowheads indicate in each panel the position of the 1500, 400, and 50 bp fragments of the FastRuler Low Range marker (Fermentas). L (size standard in bp).
