Comparison of Next-Generation Sequencing Systems

Table 4

The comparison between PGM and MiSeq.

	PGM	MiSeq
Output	10 MB–100 MB	120 MB–1.5 GB
Read length	~200 bp	Up to 2 × 150 bp
Sequencing time	2 hours for 1 × 200 bp	3 hours for 1 × 36 single read
		27 hours for 2 × 150 bp pair end read
Sample preparation time	8 samples in parallel, less than 6 hrs	As fast as 2 hrs, with 15 minutes hand on time
Sequencing method	semiconductor technology with a simple; sequencing chemistry	Sequencing by synthesis (SBS)
Potential for development	Various parameters; (read length, cycle time, accuracy, etc.)	Limited factors, major concentrate in flowcell surface size, insert sizes, and how to pack cluster in tighter
Input amount	μg	Ng (Nextera)
Data analysis	Off instrument	On instrument