256178.fig.002a
(a)
256178.fig.002b
(b)
Figure 2: Apoptosis formation by DIM in Colo 320 and HGT116 cell lines. Cells were treated with DIM 60 μM for 72 hours. DNA was extracted and separated on a 1.5% Agarose gel. The data shown were repeated three times (a). Morphologic changes, denoting apoptotic cells were shown by cell morphological changes. Cells were incubated in the presence of 60 μM DIM for 48 hours and stained with DAPI. Morphologic changes are shown by arrows (b). The data shown were repeated three times.