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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 260983, 13 pages
CD73 Is Critical for the Resolution of Murine Colonic Inflammation
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, C5 149 VMC, Ithaca, NY 14853, USA
2Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Received 14 May 2012; Revised 7 July 2012; Accepted 11 July 2012
Academic Editor: Linda F. Thompson
Copyright © 2012 Margaret S. Bynoe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Supplementary Figure S1: H&E staining of paraffin embedded colons from rag–/– mice 45 days post cell transfer: i) WT CD45RB+ donor, ii) CD73–/– CD45RB+ donor, iii) WT CD45RB+ & WT CD25+ donors, iv) CD73–/– CD45RB+ and CD73–/– CD25+ donors.
Supplementary Figure S2: Colons were isolated from WT and CD73–/– mice at different time points during treatment with 3% DSS in drinking water or after DSS removal. Equal amounts of protein were loaded for each sample/group. Expression level of the p65 subunit of NF-kappa-B was analyzed by Western blot using specific mAb (one of two experiments).
Supplementary Figure S3: Colons from CD73–/– or WT mice with IBD (day 8 of IBD) were analyzed for mRNA expression of all mouse TLRs (TLR1-11) by real-time PCR. Data is presented as fold increase in expression level compared to WT.