Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens

Figure 6

Amplification results of S. typhimurium from homogenized infected organs; 1-2 = spleen and liver after 7 days; 3-4 = spleen and liver after 10 days; 5-6 = cecum after 10 and 35 days; 7 = negative control; 8 = positive control. M = 1 kb marker (Fermentas, Germany). Detection of 700 bp amplification product was found as specific polymorphic marker for S. typhimurium. Arrows indicate 500 bp and 1,000 bp DNA fragments.