Treatment of Mycoplasma Contamination in Cell Cultures with Plasmocin
Table 2
Treatment of Mycoplasma-Positive Cell Lines with Various Antibiotics.
Mycoplasma
Cell line
Species
Tissue/cell type
Plasmocin
BM-Cyclin
Baytril
Other AB
A. laidlawii
HELA
Human
Cervix carcinoma
C
C
KYAE-1
Human
Esophagus adenocarcinoma
C
C
Mz-ChA-1
Human
Gall bladder
C
C
C
Mz-ChA-2
Human
Gall bladder
C
C
R
PLT-2
Human
Leukemia
R → C
C
M. arginini
A-431
Human
Epidermoid carcinoma
C
C
C (MycoZap)
BL-100
Human
Lymphoma
C
C
C
Cell line 1
Syrian hamster
Embryo fibroblasts
C
C
Cell line 3
Mouse
Hybridoma
C
MYL
Human
Leukemia
R
C
R
TA-5
Mouse
Hybridoma
C
R
C (MycoZap)
M. arginini + M. fermentans
JEKO-1
Human
Lymphoma
D
C
M. fermentans
CAL-1
Human
Leukemia
C
C
D
EB-3
Human
Lymphoma
C
C
C (MycoZap)
FPR2+HL60
Human
Leukemia
C
C
C
C (Ciprobay)
HBL-2
Human
Lymphoma
C
C
C
JOK-1
Human
Lymphoma
C
D
C
KOPN-K
Human
Leukemia
D
D
C
LAN-6
Human
Neuroblastoma
C
C
C
NALM-16
Human
Leukemia
C
C
NBL-S
Human
Neuroblastoma
C
C
NCEB-1
Human
Lymphoma
C
C
R
NK-9
Human
Leukemia
C
C
C (MycoZap)
RAMOS
Human
Lymphoma
D → C
C
RI-1D
Human
Lymphoma
C
C
C
SACHI
Human
Leukemia
R → C
C
R
R (MRA)
SKK-1
Human
Leukemia
C
C
C
M. fermentans + M. hyorhinis
DND-39
Human
Lymphoma
C
D
M. fermentans + M. orale
LS
Human
Neuroblastoma
C
C
C
M. hyorhinis
B6
Pig
Intestinal cells
D
C
B10XI
Pig
Intestinal cells
C
C
C
Cell line 4
Mouse
Hybridoma
C
Cell line 5
Mouse
Hybridoma
C
Cell line 6
Mouse
Hybridoma
C
C
CIEB
Calf
Colon
R
C
R
CLAB
Pig
Colon
R
C
R
HBL-2
Human
Lymphoma
C
C
C
HEK-293
Human
Embryo kidney
C
C
C
MEF clone 1
Mouse
Embryo fibroblasts
C
R
C
MEF clone 2
Mouse
Embryo fibroblasts
C
R
C
MEL-IM
Human
Melanoma
R
C
R
PANC-02
Mouse
Pancreas carcinoma
R
C
R
PC-12 clone 1
Rat
Pheochromocytoma
C
C
C
PC-12 clone 2
Rat
Pheochromocytoma
C
C
C (Ciprobay)
PC-MDS
Human
Leukemia
C
C
C
PSI
Pig
Colon
R
C
R
SVG-A
Human
Glial cells
C
C
C
WSU-DLCL-2
Human
Lymphoma
R → C
R
R (MRA)
M. orale
Cell line 2
Syrian hamster
Embryo fibroblasts
C
C
GI-ME-N
Human
Neuroblastoma
C
C
H-1963
Human
Non-small-cell lung carcinoma
C
C
HCC-44
Human
Non-small-cell lung carcinoma
C
LAN-1
Human
Neuroblastoma
C
C
NMB
Human
Neuroblastoma
C
C
Not determined
CHO clone 1
Chinese hamster
Ovary
C
C
C
CHO clone 2
Chinese hamster
Ovary
C
C
C
JW-8
Mouse
Hybridoma
C
C
C
U-266
Human
Leukemia
C
C
C
Outcome of treatment: C: cured; D: dead; R: resistant; →: back-up (untreated) mycoplasma-positive cells were treated in a second attempt. AB: antibiotics (as indicated in table); cell lines 1-6: cell lines received for elimination of mycoplasma as service.
