In Vivo Clearance of Alpha-1 Acid Glycoprotein Is Influenced by the Extent of Its N-Linked Glycosylation and by Its Interaction with the Vessel Wall

Figure 6

Either systemic hyaluronidase treatment or AGP administration reduces HABP binding to the vessel wall of mouse liver sinusoids in vivo. Mice were treated with hyaluronidase (Hy’ase; (b) and (e)) or without hyaluronidase (no treatment; (a) and (d)) after injection of either fluorescent HABP complex or fluorescent GS-1B₄ and visualization of liver sinusoids by confocal intravital microscopy. (c) Is an image of an experiment in which AGP intravenous injection was substituted for hyaluronidase. (f) (Mean of 𝑛=3±SD) quantifies the intrasinusoidal HABP fluorescence in the experiment shown in (c) and in three additional replicates; each replicate is the mean of four intravascular samples within microscopic fields similar to that seen in (c); *signifies 𝑃<0.05 by unpaired 𝑡-test, Welch corrected.