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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 301931, 8 pages
http://dx.doi.org/10.1155/2012/301931
Research Article

Enhancement of Recombinant Human Endostatin on the Radiosensitivity of Human Pulmonary Adenocarcinoma A549 Cells and Its Mechanism

Department of Radiology, Lianyungang First People's Hospital, Jiangsu, Lianyungang 222002, China

Received 26 January 2012; Accepted 27 April 2012

Academic Editor: Andre Van Wijnen

Copyright © 2012 Xiao-dong Jiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We observed the effects of endostar on the radiosensitivity of pulmonary adenocarcinoma A549 cells and found that endostar inhibited A549 cell growth under normoxia and hypoxia in time and dose-dependent manners; the 𝐷 0 and 𝐷 𝑞 values in control and endostar groups were (1.36 and 1.30) versus (1.019 and 1.015) under normoxia and (1.693 and 1.39) versus (2.453 and 1.026) under hypoxia, respectively; SER was 1.04 under normoxia and 1.22 under hypoxia in endostar group; under normoxia, the apoptosis rates in control, radiotherapy, endostar and combination groups were 1 5 . 9 ± 0 . 5 7 %, 4 2 . 7 ± 0 . 3 7 %, 1 9 . 9 ± 0 . 4 8 %, and 4 1 . 5 ± 0 . 3 8 %, respectively, with no significant difference between combination and radiotherapy groups; there was significant difference in G2/M phase cells between combination and radiotherapy groups ( 𝑃 = 0 . 0 2 8 ); under hypoxia, the apoptosis rates in the four groups were 1 6 . 7 ± 0 . 6 7 %, 3 0 . 1 ± 0 . 9 5 %, 2 6 . 7 ± 0 . 6 2 %, and 3 6 . 3 ± 0 . 7 1 %, respectively, with significant difference between combination and radiotherapy groups; G2/M phase cells were higher in combination group than radiotherapy group ( 𝑃 = 0 . 0 0 0 ); G2/M phase cells were higher in hypoxic combination group than in normoxic combination group ( 𝑃 = 0 . 0 0 3 ). Based on these results, we conclude that under hypoxia, endostar can enhance the radiosensitivity of A549 cells through G2/M arrest.