TY - JOUR A2 - Gomer, Richard AU - Guo, Qianchen AU - Lu, Xuya AU - Xue, Yuan AU - Zheng, Hong AU - Zhao, Xiaotao AU - Zhao, Huajian PY - 2012 DA - 2012/10/02 TI - A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix SP - 306083 VL - 2012 AB - In vivo adhesions between cells and the extracellular matrix play a crucial role in cell differentiation, proliferation, and migration as well as tissue remodeling. Natural three-dimensional (3D) matrices, such as self-assembling matrices and Matrigel, have limitations in terms of their biomechanical properties. Here, we present a simple method to produce an acellular human amniotic matrix (AHAM) with preserved biomechanical properties and a favorable adhesion potential. On the stromal side of the AHAM, human foreskin fibroblasts (HFFs) attached and extended with bipolar spindle-shaped morphology proliferated to multilayer networks, invaded into the AHAM, and migrated in a straight line. Moreover, αV integrin, paxillin, and fibronectin were observed to colocalize after 24 h of HFF culture on the stromal side of the AHAM. Our results indicate that the AHAM may be an ideal candidate as a cell-matrix adhesion substrate to study cell adhesion and invasion as well as other functions in vitro under a tensile force that mimics the in vivo environment. SN - 2314-6133 UR - https://doi.org/10.1155/2012/306083 DO - 10.1155/2012/306083 JF - Journal of Biomedicine and Biotechnology PB - Hindawi Publishing Corporation KW - ER -