Research Article

A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix

Figure 3

Morphology of HFFs and quantification of cell length, width, and spread area on the indicated substrates. ((a) and (b)) β-actin was detected by immunofluorescence staining to visualize the cytoskeleton and morphology at 24 h after plating cells on glass coverslips (a) or the AHAM (b). Scale bar, 50 μm. ((c) and (d)) The cell spread area (c) and axial ratio (d) were calculated on the indicated substrates after immunofluorescence staining. Cells on coverslips were nearly twice as large in terms of their cell spread area, compared with that of cells cultured on the AHAM. Error bars indicate standard error (* 𝑃 < 0 . 0 5 and ** 𝑃 < 0 . 0 0 1 ).
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