Research Article

A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix

Figure 5

Confocal images of indirect immunofluorescence staining of HFFs on the AHAM, glass coverslips, and Matrigel. Triple labeling of αV integrin (green) ((a)–(c)), paxillin (red) ((d)–(f)), and fibronectin (blue) ((g)–(i)) was performed. Colocalization was observed as white bands (white arrows) only within the AHAM (l), and not with cell adhesions on coverslips (j) or Matrigel (k). Colocalization of αV integrin and paxillin was detected as yellow bands (red arrows) in regions without fibronectin only within the AHAM (l). Scale bar, 10  𝜇 m.
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