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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 313814, 7 pages
Review Article

Sarcomere Imaging by Quantum Dots for the Study of Cardiac Muscle Physiology

1Department of Cell Physiology, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo 105-8461, Japan
2Department of Physics, Waseda University, Tokyo 169-8555, Japan
3Department of Physics, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan

Received 20 October 2011; Accepted 7 January 2012

Academic Editor: P. Bryant Chase

Copyright © 2012 Fuyu Kobirumaki-Shimozawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Video 1: Movie showing ADP-SPOC in a skinned cardiomyocyte treated with anti-α-actinin antibody-QDs. Excited by blue light. Speed, 31 fps. See Figure 1 for the sarcomere structure and antibody conjugated-QDs.

Supplemental Video 2: Same myocyte as in Supplemental Video 1 but observed under the bright field. Speed, 31 fps.

Supplemental Video 3: Movie showing Ca2+ sparks at rest without stimulation followed by a Ca2+ wave propagating through a rat ventricular myocyte. Ca2+ was detected with Fluo-4 at 25°C, based on (25). Bar, 10 μm. Speed, 31 fps.

  1. Supplementary Video 1
  2. Supplementary Video 2
  3. Supplementary Video 3