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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 341479, 7 pages
http://dx.doi.org/10.1155/2012/341479
Research Article

Induction of Apoptosis and Cell Cycle Arrest in Human Colorectal Carcinoma by Litchi Seed Extract

1Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu 30015, Taiwan
2Department of Biotechnology, Yuanpei University, Hsinchu 30015, Taiwan
3Department of Anesthesiology, China Medical University Hospital, Taichung 40447, Taiwan
4Department of Natural Resources and Environmental Studies, National Dong Hwa University, Hualien 97401, Taiwan
5Department of Surgery, Cheng-Ching Hospital, Chung-Kang Branch, No. 118 SEC.3 Chung Kang Road, Taichung City 407, Taiwan
6Department of Surgery, National Taiwan University Hospital, Taipei 10048, Taiwan

Received 13 May 2012; Accepted 29 July 2012

Academic Editor: Rudi Beyaert

Copyright © 2012 Chih-Ping Hsu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The Litchi (Litchi chinensis) fruit products possess rich amounts of flavanoids and proanthocyanidins. Its pericarp has been shown to inhibit breast and liver cancer cell growth. However, the anticolorectal cancer effect of Litchi seed extract has not yet been reported. In this study, the effects of polyphenol-rich Litchi seed ethanol extract (LCSP) on the proliferation, cell cycle, and apoptosis of two colorectal cancer cell lines Colo320DM and SW480 were examined. The results demonstrated that LCSP significantly induced apoptotic cell death in a dose-dependent manner and arrested cell cycle in G2/M in colorectal carcinoma cells. LCSP also suppressed cyclins and elevated the Bax : Bcl-2 ratio and caspase 3 activity. This study provides in vitro evidence that LCSP serves as a potential chemopreventive agent for colorectal cancer.