Figure 3: Immunoblots of cell cycle-controlling proteins in LCSP-treated CRC cells. Cells were treated with increasing concentrations of LCSP as indicated and then incubated at 37°C for 24 h. Cell protein lysates from Colo320DM (a) and SW480 (b) cells were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted to show cyclin D1, cyclin A, and cyclin B1 levels, and the beta-actin level as a loading control.