Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad

Table 1

11.5 dpc Mouse embryos used to analysis of Sry knockdown. GFP expression was assessed macroscopically by examining whole urogenital systems under a dissecting microscope equipped with fluorescence optics. The density of GFP fluorescence was classified as low, medium, or high. Feminization was assessed by gonadal histology, immunofluorescent marker expression, or quantitative RT–PCR.

Plasmid treated at the 9.5 dpc	Number of embryos at 11.5 dpc (both sexes)	Number of embryos (both sexes) showing high GFP expression	Total number of feminized males/males with high GFP
5% glucose solution	𝑛=60	0	0/27
pSilencer4.1/EGFP	𝑛=78	40	0/18
pSilencer4.1/Sry565	𝑛=320	56	8/27