Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation

Table 1

The Plackett-Burman experimental design matrix for screening culture condition factors affecting biosurfactant production by B. subtilis SPB1 strain.

Run order	Temperature	Moisture	Initial	Inoculum size	Inoculum	Biosurfactant
	(°C)	(%)	pH	(CFU/mL)	age (h)	mg/g
	𝐴	𝐵	𝐶	𝐷	𝐸
1	45	100	9	10^6	24	16.3
2	25	100	9	10^8	4	13.9
3	25	50	9	10^8	24	14.1
4	45	50	2	10^8	24	16.3
5	25	100	2	10^6	24	12.7
6	45	50	9	10^6	4	11.5
7	45	100	2	10^8	4	17.5
8	25	50	2	10^6	4	9.2