Frequency of TLR 2, 4, and 9 Gene Polymorphisms in Chinese Population and Their Susceptibility to Type 2 Diabetes and Coronary Artery Disease

Table 2

Primers, PCR conditions, and restriction enzymes used for genotyping TLRs gene.

SNPs		Primer sequence 5′-3′	AT	PCR product	RE	Expected product
TLR2	Arg677Trp	F: TAT GGT CCA GGA GCT GGA GA	60°C	430 bp	AciI	Wt: 228 bp
	Arg753Gln	R: TGA CAT AAA GAT CCC AAC TAG ACA A				Arg677Trp: 283 bp; Arg677Trp: 375 bp
TLR4	Arg299Gly	F: GAT TAG CAT ACT TAG ACT ACT ACC TCC ATG	55°C	249 bp	NcoI
		R: GAT CAA CTT CTG AAA AAG CAT TCC CAC				Wt: 249 bp; Mt: 223 bp
	Thr399Ile	F: GGT TGC TGT TCT CAA AGT GAT TTT GGG AGA A	60°C	406 bp	HinfI
		R:A CCT GAA GAC TGG AGA GTG AGT TAA ATG CT				Wt: 406 bp; Mt: 377 bp
TLR9	T-1237C	F: ATG GGA GCA GAG ACA TAA TGG A	62°C	135 bp	BstNI
		R: CTG CTT GCA GTT GAC TGT GT				Wt: 108 bp; Mt: 60 bp 48 bp
	T-1486C	F: TCC CAG CAG CAA CAA TTC ATT A	62°C	499 bp	AflII
		R: CTG CTT GCA GTT GAC TGT GT				Wt: 327 bp; Mt: 499 bp

F: forward primer; R: reverse primer; AT: annealing temperature; RE: restriction enzyme.