Table 1: List of SYBR Green screening methods developed and validated at WIV-ISP.

Method nameTargetFragment size (bp)Reference

RBCLRibulose-1,5-biphosphate carboxylase oxygenase 95[73]

Plant taxon-specific methods

LEC Lectin gene of soybean (Glycine max L.)81[74]
ADH Alcohol dehydrogenase I gene from maize (Zea mays L.)83[73]
CRU Cruciferin gene from oilseed rape (Brassica napus)85[73]
PLD Phospholipase D gene from rice (Oryza sativa)80[73]
SAD1 Stearoyl-acyl carrier protein desaturase gene of cotton (Gossypium genus)107[75]
GLU3 Glutamine synthetase gene from sugar beet (Beta vulgaris)118[31]

Generic element-specific methods

p35SPromoter of the 35 S cauliflower mosaic virus75[28]
tNOSTerminator of the nopaline synthase gene69[28]
pFMVPromoter of the figworth mosaic virus79[76]
pNOSPromoter of the nopaline synthase gene75[76]
t35STerminator of the cauliflower mosaic virus107in-house

GM element-specific methods

cryIAb/Ac Gene encoding the Bacillus thuringiensis δ-endotoxin (insect resistance)73[68]
cry3Bb Gene encoding the Bacillus thuringiensis δ-endotoxin (insect resistance)105in-house
pat Phosphinotricin-N-acetyltransferases gene from Streptomyces viridochromogenes 109[68]
bar Phosphinotricin-N-acetyltransferases gene from Streptomyces hygroscopicus 69[68]
epsps 5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain CP4108[68]

p35S discriminating method

CRTReverse transcriptase gene from the cauliflower mosaic virus94in-house