Review Article
Heterologous Expression of Plant Cell Wall Degrading Enzymes for Effective Production of Cellulosic Biofuels
Table 1
Summary of expression of plant cell wall degrading enzymes in Z. mobilis.
| | PCDE | Source organism | Gene Bank ID | M.W (kDa) | Promoter | Secretion signal peptide | Extracellular portion (%) | Periplasmic portion (%) | Reference |
| | Endo--1,4-glucanase | A. cellulolyticus | AAA75477.1 | 60.7 | | Z. mobilis PhoC | ~20% | ~30% | [42] | | A. cellulolyticus | AAA75477.1 | 60.7 | | Z. mobilis ORF ZM 0331 | ~15% | ~25% | [42] | | A. cellulolyticus | ABK52392.1 | 41.3 | | Z. mobilis PhoC | 13% | 26% | [42] | | A. xylinum | BAA03797.1 | 24 | Native | Native | <1% | 75% | [43] | | B. subtilis | NA | NA | Native | Native | NA | NA | [44] | | C. uda CB4 | AAA23090.1 | 40.7 | Z. mobilis unidentified gene | Native | NA | NA | [45] | | E. chrysanthemi | CAA68604.1 | 46.4 | Native | Native | ~40% | ~80% | [46] | | E. cloacae | ABP62583.1 | 40.3 | Native | Native | ~7.6% | ND | [47] | | P. cellulosa | NA | NA | gene on pSUP104 | Native | NA | NA | [48] | | X. albilineans | NA | NA | Native | Native | NA | NA | [49] |
| | -Glucosidase | R. albus | CAA33461.1 | 104.2 | Z. mobilis gfo gene | Z. mobilis Gfo | 4.7% | 61% | [50] | | R. albus | CAA33461.1 | 104.2 | | Z. mobilis Gln | 11.2% | 34.3% | [50] | | X. albilineans | NA | NA | Native | Native | NA | NA | [49, 51] |
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*Chloramphenicol acyltransferase; NA: not available; ND: not detected.
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