Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept

Table 1

Targeted BAC clone sequencing. Assembly statistics, including N50 contig size, max contig, and BLASTn 𝐸-value correspondence with SNP query sequence.

Positive BAC clone address	SNP target	BAC Assembly Statistics						BLASTn 𝐸-value
		No. of reads	No. of bases	Contigs (>500 bp)	N50 contig Size (bp)	Max contig (bp)	Number of bases
16O4	AM18081	28417	11346335	14	52842	58444	187937	9.65𝐸−101
124I	AM20886	58578	21913991	15	25282	57023	181556	6.68𝐸−111
3L14	AM21120	12523	4717010	8	124794	124794	235849	5.36𝐸−109
6M9	AM21773	38937	14568922	15	23276	54030	125585	1.90𝐸−113
13G11	AM22193	13529	4999229	15	30565	40572	206077	7.58𝐸−111
15O11	AM23006	9887	3722293	9	30149	66008	184367	6.79𝐸−111
9N2	AM23895	26614	9798881	4	48808	56894	181431	9.33𝐸−101
3L19	AM25953	30301	11263931	7	147194	147194	164652	6.10𝐸−111
Average				10.9	60364	75620	183432