Proteomic Profiling of the Dioxin-Degrading Bacterium Sphingomonas wittichii RW1

Table 3

Highly abundant proteins identified as unchanged in S. wittichii RW1 during growth on dibenzofuran or acetate. The spots were identified on a minimum of 6 gel images.

Master number^a	Protein accession^b	Gene locus	Protein name	Average ratio^c	𝑡-test^d
916	148556048	Swit_3144	TonB-dependent receptor	−1.33	0.23
929	148553835	Swit_0912	4-Oxalocrotonate decarboxylase	−1.26	0.25
256	148556278	Swit_3376	Chaperonin GroEL	−1.21	0.5
453	148553821	Swit_0898	Phenylpropionate dioxygenase, ferredoxin reductase subunit	−1.17	0.5
741	148553833	Swit_0910	Alpha/beta hydrolase fold	−1.08	0.9
933	148555961	Swit_3055	Meta-cleavage product hydrolase	−1.03	0.85
932	148553776	Swit_0853	OmpA/MotB domain	−1.00	0.93
749	148553834	Swit_0911	4-Oxalocrotonate decarboxylase	1.09	0.3
927	148555704	Swit_2794	Opacity protein and related surface antigen-like protein	1.09	0.82
648	148550878	Swit_4921	3-Keto-5-aminohexanoate cleavage enzyme	1.15	0.45
177	148555643	Swit_2731	Aconitate hydratase 1	1.23	0.44
479	148550877	Swit_4920	FAD-dependent pyridine nucleotide-disulphide oxidoreductase	1.57	0.5
937	148550856	Swit_4897	Dioxin dioxygenase, alpha subunit	1.59	0.38

^a Arbitrary identifier for spot location (see Figure 2).
^bNCBI gi∣ number.
^cUsing internal standard as 1.0, >1 is an increase and <1 is a decrease in abundance.
^dStudent’s t-test comparing spot intensity for acetate versus dibenzofuran grown cells.