Research Article
The Osteogenic Study of Tissue Engineering Bone with BMP2 and BMP7 Gene-Modified Rat Adipose-Derived Stem Cell
Table 1
Primers used for real-time PCR analysis.
| | Gene | Primers | Annealing temperature (°C) |
| | ALP | Forward: 5′-AGGCAGGATTGACCACGG-3′ | 58 | | Reverse: 5′-TGTAGTTCTGCTCATGGA-3′ | | OPN | Forward: 5′-GACGGCCGAGGTGATAGCTT-3′ | 63 | | Reverse: 5′-CATGGCTGGTCTTCCCGTTGC-3′ | | OC | Forward: 5′-AAAGCCCAGCGACTCTC-3′ | 59 | | Reverse: 5′-CTAAACGGTGGTGCCATAGAT-3′ | | GAPDH | Forward: 5′-AACCCATCACCATCTTCCAGG-3′ | 60 | | Reverse: 5′-GCCTTCTCCATGGTGGTGAA-3′ |
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