Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro

Table 1

Primer data.

Gene	Accession number	Fwd primer (5′–3′)	Rev. primer (5′–3′)	Amplicon size	PCR efficiency
arp (ref. gen)	AY255630	GAAAATCATCCAATTGCTGGATG	CTTCCCACGCAAGGACAGA	101	1,813
ef1ab (ref. gene)	BG933853	TGCCCCTCCAGGATGTCTAC	CACGGCCCACAGGTACTG	59	2,013
fatp	CA373015/AF023258	TGGGAGCTTGTGGGTTCAA	ACTTTCATGAGGCGGATTGG	64	2,243
actb	BG933897	CCAAAGCCAACAGGGAGAAG	AGGGACAACACTGCCTGGAT	92	2,007
gst	BQ036247	ATTTTGGGACGGGCTGACA	CCTGGTGCTCTGCTCCAGTT	81	2,07
tuba	BT049768	GAGCCAGCCAATCAGATGGT	TGCGCTTGGTCTTGATTGTG	110	2,009
gpx	BQ036408	TTCTCCACCACACTGGGATCA	GGAAATGGCATCAAGTGGAATT	101	2,011
hsp-70	BG933934	CCCCTGTCCCTGGCTATTG	CACCAGGCTGGTTGTCTGAGT	121	1,895
cox2	AJ238307	CGTCCTGAGGCAGGAGCAT	TGAGGCGTGTGGTCTGGAA	62	2,18
cpt1	AM230810	CTTTGGGAAGGGCCTGATC	CATGGACGCCTCGTACGTTA	121	1,917
bcl-x	NM_001141086	GCCTGGACGCAGTGAAAGAG	GGACGGCGTGATGTGTAGCT	107	2,127

Full gene names: acidic ribosomal protein (arp), elongation factor 1 alpha b (ef1ab), fatty acid transport protein (fatp), β-actin (actb), glutathione-s-transferase (gst), tubulin α (tuba), glutathione peroxidase (gpx), heat shock protein 70 (hsp-70), cyclooxygenase 2 (cox2), carnitine palmitoyltransferase 1 (cpt1), and B-cell lymphoma x (bcl-x).