Identification of Pigment Epithelium-Derived Factor Protein Forms with Distinct Activities on Tumor Cell Lines
Anion-exchange Column Chromatography of PEDF. Chromatogram of PEDF with a POROS HQ column attached to BioCad700, running buffer of 50 mM Tris-HCl, pH 8, and a flow rate of 3 mL/min. Elution was with 25 column volumes of a linear gradient from 100 to 300 mM NaCl. On the chromatogram, blue indicates the O.D. of the sample read at 280 nm, magenta indicates the conductivity. Resolution of load and peak fractions by IEF is shown in the inset.