Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation
Figure 7
Parent cells (a and c) and B-7 cells (b and d) were inoculated into the hind flank of nude mice (left, parent cells; right, B-7 cells) with (FAC+) or without (FAC−) FAC supplementation in the drinking water. The tumors were collected for histological examination at the end of 3 weeks. (a and b) HE staining showed overexpression of FTH1 did not cause obvious pathological changes. (c and d) Immunohistochemistry demonstrated overexpression of FTH1 was easily detected in B-7-cell-induced tumors but not in parent-cell-induced tumors. (e) Luciferase activity detected by using bioluminescence imaging technology in vivo. Luciferase activity in B-7 cell induced tumors was increased up to 106 photons/sec, but that was not significantly changed in the parent-cell-induced tumors as compared to the background activity (<300 photons/sec). Representative image from mice in the presence of FAC. (f) ICP-MS analysis of Fe content measured in parts per million (ppm) dry weight of parent cells or B-7 cell induced tumors. Data were expressed as mean ± SD from 6 experiments. (*).
