Bacterial Artificial Chromosome Libraries of Pulse Crops: Characteristics and Applications

Table 1

Bacterial artificial chromosome (BAC) libraries of pulse crops.

Species (common name)	Genotype	G-size^a; Mb	Vector	R-site^b	No. of clones	A.I-size^c; kb	Cov^d	Use^e	Ref.^f
Phaseolus vulgaris L. (Common bean)	Sprite	636	pECSBC4	RcoRI	33,792	100	5.3	Ph.M.	[13]
	Bat 93		pIndigoBac5	HindIII	110,592	125	21.7	Evolution	[14]
	G21245		pIndigoBac5	HindIII	55296	105	9.2	Evolution	[14]
	G02771		pIndigoBac5	HindIII	55296	139	12.1	Evolution	[14]
	G12949		pIndigoBac5	HindIII	30720	135	6.5	cloning	[15]
	G19833		pIndigoBac536	HindIII	55296	145	12.6	Ph.M BES	[16]
	HR45		pIndigoBac5	HindIII	33,024	107	5.5	Ph.M.Cloning	[17]
	HR67		BiBAC2	BamHI	22,560	300	10.6	Cloning	[18]
	OAC-Rex		BiBAC2	BamHI	31,776	150	7.5	Cloning	[18]
	G02333		pBeloBac11	HindIII	24,960	125	4.9	Cloning	[19]
P. lunatus L. (Lima bean)	Henderson	636	pIndigoBac5	HindIII	55,296	110	9.6	Evolution	[14]
Vigna radiate (Mungbean)	ACC41	587	pBeloBacII	HindIII	6,912	107	1.3	Mar-dev.	[12]
	ATT3640		pBeloBacII	BamHI	11,904	113	2.3	Mar-dev.	[12]
V. unguiculata (Cowpea)	IT97K-499-35	611	pCC1	HindIII	36,864	150	9.1	Cloning	[20]
	IT97K-499-35		pCC1	MboI	36,864	130	7.8	Cloning	[20]
Cicer arietinum (Chickpea)	FLIP84-92C	758	pCLDO4541	HindIII	23,780	100	3.1	Cloning	[21]
	Hadas		pIndigoBac5	HindIII	14,976	121	2.4	Mar-dev.	[22]
	Hadas		pCLD04541	BamHI	23,040	145	4.4	Mar-dev.	[22]
	Hadas		pECBAC1	MboI	22,272	130	3.8	Ph.M	[23]
	Hadas		pCLD04541	HindIII	38,400	142	7.2	Ph.M.	[23]
Pisum sativum (Pea)	PI269818	4397	pCLD04541	HindIII	55,680	105	1.3		[24]
	PI269818	4397	pIndigoBac5	HindIII	65,280	105	2.2		[24]
Cajanus cajan L. (Pigeonpea)	Asha	831	pCC1BAC	HindIII	34,560	120	5.0	Mar-dev.	[25]
	Asha		pCC1BAC	BamHI	34,560	115	4.8	Mar-dev.	[25]
Lupinus Angustifolius (Lupin)	Sonet	905	pIndigoBac5	HindIII	55,296	100	6.1	Karyotype	[26]

^aG-size: Genome size; genome size estimates based on Arumuganathan, and Earle [27] for all species but pigeonpea, which was based on Greilhuber and Obermayer, [28].
^bR-site: Restriction site.
^cA.I-size: Average insert size.
^dCov: Genome coverage; for consistency of comparisons, genome coverage was calculated using the genome estimates in Table 1, which may be different from the cited literature.
^ePh.M, physical mapping; BES: BAC end sequences; Mar-dev: marker development.
^fMost of the libraries, if not all, may be accessed through collaborative research by contacting the authors who developed the library.