Effects of Norepinephrine and Acetylcholine on the Development of Cultured Leydig Cells in Mice

Table 1

Primer sequences.

	Primer sequence	Product size (bp)	Annealing temp. (^°C)	Reference
β1AR	F: CCGCTGCTACCACGACCCCAAG	441	60	[11]
	R: AGCCAGTTGAAGAAGAGCAAGAGGCG
β2AR	F: GGTTATCGTCCTGGCCATCGTGTTTG	468	61	v.s.
	R: TGGTTCGTGAAGAAGTCACAGCAAGTCTC
β3AR	F: TCTAGTTCCCAGCGGAGTTTTCATCG	337	64	v.s.
	R: CGCGCACCTTCATAGCCATCAAACC
α1A	F: GGACAAGTCAGACTCAGAGCAAGTGACG	404	61	v.s.
	R: TATAGCCCAGGGCATGCTTGGAAGAC
α1B	F: TTTCATGAGGACACCCTCAGCAGTACC	452	61	v.s.
	R: CTGCCGCTGTCATCCAGAGAGTCC
α1D	F: CGCCAAAGGAAATCCAGGGACAC	617	61	v.s.
	R: CAGAGCGGAACTTATGGGACAGG
M1	F: GTAAGGTGCCTGCCATCCAATC	417	58	[12]
	R: CGCAGCTCACTTTCTGCATTGT
M2	F: TGTCAGCAATGCCTCCGTTATG	480	58	v.s.
	R: GCCTTGCCATTCTGGATCTTG
M3	F: GGTGTGATGATTGGTCTGGCTTG	497	62	v.s.
	R: GAAGCAGAGTTTTCCAGGGAG
M4	F: TCAAGAGCCCTCTGATGAAGCC	477	62	v.s.
	R: AGATTGTCCGAGTCACTTTGCG
M5	F: GCTGACCTCCAAGGTTCCGATTC	485	54	v.s.
	R: CCGTCAGCTTTTACCACCAAT
3β-HSD	F: ACTGCAGGAGGTCAGAGCT	565	55	[13]
	R: GCCAGTAACACACAGAATACC
PCNA	F: TGGAATCCCAGAACAGG	287	55	[14]
	R: ACAGTGGAGTGGCTTTT
GAPDH	F: AAACCCATCACCATCTTCCAG	361	54	[15]
	R: AGGGGCCATCCACAGTCTTCT

F: forward; R: reverse.