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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 504037, 10 pages
Research Article

Long-Term Left Ventricular Remodelling in Rat Model of Nonreperfused Myocardial Infarction: Sequential MR Imaging Using a 3T Clinical Scanner

1MRC/UCT Medical Imaging Research Unit, Department of Human Biology, University of Cape Town, Observatory 7925, South Africa
2Cardiovascular Research Unit, Chris Barnard Division of Cardiothoracic Surgery, University of Cape Town, Observatory 7925, South Africa
3Division of Radiodiagnosis, Stellenbosch University, Matieland 7602, South Africa
4Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA 02129, USA
5Department of Radiology, Harvard Medical School, Boston, MA 02115, USA
6Centre for Research in Computational and Applied Mechanics, University of Cape Town, Rondebosch 7701, South Africa
7Centre for High Performance Computing, Rosebank 7700, South Africa

Received 6 March 2012; Accepted 11 June 2012

Academic Editor: M. Piacentini

Copyright © 2012 Muhammad G. Saleh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To evaluate whether 3T clinical MRI with a small-animal coil and gradient-echo (GE) sequence could be used to characterize long-term left ventricular remodelling (LVR) following nonreperfused myocardial infarction (MI) using semi-automatic segmentation software (SASS) in a rat model. Materials and Methods. 5 healthy rats were used to validate left ventricular mass (LVM) measured by MRI with postmortem values. 5 sham and 7 infarcted rats were scanned at 2 and 4 weeks after surgery to allow for functional and structural analysis of the heart. Measurements included ejection fraction (EF), end-diastolic volume (EDV), end-systolic volume (ESV), and LVM. Changes in different regions of the heart were quantified using wall thickness analyses. Results. LVM validation in healthy rats demonstrated high correlation between MR and postmortem values. Functional assessment at 4 weeks after MI revealed considerable reduction in EF, increases in ESV, EDV, and LVM, and contractile dysfunction in infarcted and noninfarcted regions. Conclusion. Clinical 3T MRI with a small animal coil and GE sequence generated images in a rat heart with adequate signal-to-noise ratio (SNR) for successful semiautomatic segmentation to accurately and rapidly evaluate long-term LVR after MI.